Alkaline Phosphatase Activity Assay Catalog: MA-0109

Catalog MA-0109

Size 500 wells

Sample Type:  Cell Extracts, Tissue lysates, Serum, Other Biological Fluids.

Species All

Method of Detection Colorimetric, OD 405 nm

Assay Type Quantitative

Application A plate based colorimetric assay to measure the presence and activity of alkaline phosphatase enzyme in various sample types.

Sensitivity >10 µU

Storage Conditions 4°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Alkaline phosphatase (ALP) is an enzyme that catalyzes the hydrolysis of phosphate esters under alkaline conditions with the production of free phosphate. AkrivisBio’s ALP Activity Assay is a simple, sensitive assay to measure ALP activity in a variety of biological samples. In this assay the specific alkaline phosphatase substrate, p-nitrophenyl phosphate is hydrolyzed to phosphate ion and p-nitrophenol. P-nitrophenol formation is then monitored using a spectrophotometer at λmax 405 nm. The absorbance or color intensity is directly proportional to the alkaline phosphatase activity in the sample.