Free Fatty Acid Assay, Catalog: MA-0111

Catalog MA-0111

Size 100 wells

Sample Type  Tissue Extracts, Cell Lysate, Serum, Plasma, Urine, other biological fluids

Species All

Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A plate based colorimetric/fluorometric assay to measure the concentration of free fatty acids in samples.

Sensitivity >2 µM

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Fatty acids are an important source of energy, as structural components and as precursors to a variety of bioactive chemicals collectively referred to as oxylipins with a diverse set of biological actions, including pro- and anti-inflammatory, vasoactive, autocrine and paracrine functions. High levels of free fatty acids have been associated with conditions including obesity, diabetes, liver diseases etc. AkrivisBio’s Fatty Acid Assay is a simple and sensitive assay to measure free fatty acids in various sample types. The assay is based upon the conversion of fatty acids to CoA derivatives, which are subsequently oxidized to form color/fluorescence that is quantified at OD 570 nm or at Ex/Em = 535/587 nm respectively. The intensity of color/fluorescence is proportional to the concentration of free fatty acid in samples.