Alanine Aminotransferase (ALT) Assay, Catalog: MA-0116

Catalog MA-0116

Size 100 wells

Sample Type  Tissue Extracts, Cell Lysate, Serum, Urine, Plasma, other biological fluids

Species All

Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A plate based colorimetric/fluorometric assay to quantify Alanine aminotransferase activity in a variety of biological samples with a detection limit below 10 mU per well.

Sensitivity 10 mU/well

Storage Conditions -20°C  

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Alanine aminotransferase (ALT) is an enzyme also known as SGPT (serum glutamic pyruvic transaminase), and is found in serum and several tissues, predominantly in liver cells. High levels of ALT in the blood is an indication of liver damage or disease and is as part of a differential diagnostic test. AkrivisBio’s ALT Assay is a simple and sensitive assay to measure ALT enzyme activity in a variety of biological samples. In this assay, alanine and α-ketoglutarate are converted to pyruvate and glutamate in the presence of ALT. Pyruvate is then oxidized forming hydrogen peroxide, which is utilized by the peroxidase to oxidize ADHP thereby resulting in the formation of intense color or fluorescence measured at OD 570 nm or at Ex/Em = 535/587 nm respectively.