Urea Assay, Catalog: MA-0118

Catalog MA-0118

Size 100 wells

Sample Type  Tissue Extracts, Cell Lysate, Serum, Urine, Plasma, other biological fluids

Species All

Method of Detection Colorimetric, OD 570 nm

Assay Type Quantitative

Application A plate based colorimetric assay to measure the concentration of urea in a wide variety of samples. The assay sensitivity is 0.1-0.2 nmol of urea per well.

Sensitivity >0.5 nmol/well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Urea is a waste product formed in the liver as a result of protein metabolism and is excreted by the kidneys. It is the primary means of nitrogen excretion in mammals. Thus, measuring urea levels can provide information about kidney function or other metabolic conditions. AkrivisBio’s Urea Assay is a simple, sensitive way to measure the concentration of urea in a variety of biological samples. In this assay, urea is hydrolyzed by the urease to ammonia, which via a series of reactions results in the formation of hydrogen peroxide. Hydrogen peroxide is then utilized by the peroxidase to oxidize ADHP thereby resulting in the formation of intense color measured at OD 570 nm. The assay sensitivity is 0.1-0.2 nmol of urea per well.