Cholesterol-Cholesterol Ester Assay, Catalog: MA-0119

Catalog MA-0119

Size 100 wells

Sample Type  Tissue Extracts, Serum, Urine, Plasma, other biological fluids

Species All

Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A plate based colorimetric/fluorometric assay to quantify free, esterified or total cholesterol in biological samples.

Sensitivity <5 μg/well

Storage Conditions -20°C  

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Cholesterol is a lipid molecule essential to all animal life. It is the precursor of steroid hormones and vitamin D. It makes up about 30% of cell membranes. It is transported via lipoproteins including HDL, LDL, VLDL in the blood, as free cholesterol and as cholesteryl esters. AkrivisBio’s Cholesterol/Cholesteryl Ester Assay provides a simple, sensitive method of quantifying free, esterified or total cholesterol (cholesterol and cholesteryl esters). In this assay, cholesteryl esters are hydrolyzed to cholesterol by cholesterol esterase, which is then oxidized by cholesterol oxidase to yield hydrogen peroxide. Hydrogen peroxide is utilized by the peroxidase to oxidize ADHP thereby resulting in the formation of intense color or fluorescence measured at OD 570 nm or at Ex/Em = 535/587 nm respectively.