Pyruvate Assay, Catalog: MA-0129

Catalog MA-0129

Size 100 wells

Sample Type Tissue Extracts, Cell Lysate, Serum, Plasma, Urine, Other Biological Fluids

Species All

Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/580 nm

Assay Type Quantitative

Application A plate based colorimetric/fluorometric assay to quantitate pyruvate in the range of 1 μM - 10 mM in a variety of biological samples.

Sensitivity 1 μM-10 mM

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Pyruvate is a crucial molecule in cellular metabolism. It also serves as a key precursor for various biosynthetic pathways, including the synthesis of amino acids and fatty acids. Pyruvate’s central role in energy production and biosynthesis makes it a fundamental molecule in cellular processes. Measuring pyruvate levels can provide insights into various pathological conditions, including metabolic disorders, liver diseases, cancer etc. AkrivisBio’s Pyruvate Assay is a simple, sensitive method of quantitating pyruvate in a variety of biological sample types. In the assay, Pyruvate in the sample is oxidized by pyruvate oxidase with the formation of hydrogen peroxide. Hydrogen peroxide is then utilized by the peroxidase to oxidize ADHP forming an intense color or fluorescence measured at OD = 570 nm or at Ex/Em = 538/587 nm respectively.