Succinate Assay, Catalog: MA-0134

Catalog MA-0134

Size 100 wells

Sample Type  Cell Lysate, Tissue Extracts,

Species All

Method of Detection Colorimetric, OD 450 nm

Assay Type Quantitative

Application A plate based colorimetric assay to quantify succinate at levels below 25 μM in a variety of sample types.

Sensitivity <25 μM

Storage Conditions  -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Succinate is an important metabolite in the tricarboxylic acid cycle. Succinate has also emerged as a signaling molecule in various cellular processes, including inflammation, hypoxia responses, and regulation of gene expression. In pathological conditions such as ischemia, it accumulates, impairing mitochondrial function and promoting oxidative stress and tissue damage. AkrivisBio’s Succinate assay is a simple, sensitive method for the accurate determination of succinate in a variety of samples at levels below 25 μM. In the assay, succinate is converted to succinyl CoA with the formation of an intermediate, which then undergoes a series of reactions to generate NADH. The NADH formed then reduces nearly colorless tetrazolium to a highly colored formazan measured at OD 450 nm.