Citrate Assay, Catalog: MA-0139

Catalog MA-0139

Size 100 wells

Sample Type Cell Lysate, Tissue Extracts, Urine, Serum, Plasma, Other biological fluids

Species All

Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A plate based colorimetric/fluorometric assay to quantify citrate levels in biological samples.

Sensitivity <0.1 nmoles

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Citrate is a metabolic intermediate in the tricarboxylic acid cycle, a key metabolic pathway, responsible for generating energy in the form of ATP. It is also an allosteric modulator of fatty acid synthesis and glycolysis. Decreased citrate levels are associated with kidney stones, metabolic syndrome and prostate cancer. AkrivisBio’s Citrate Assay provides a simple, sensitive means of measuring citrate in a variety of samples with a sensitivity below 0.1 nanomoles. In the assay, citrate is converted to oxaloacetate and then to pyruvate, which is oxidized by pyruvate oxidase forming hydrogen peroxide. Hydrogen peroxide is then utilized by the peroxidase to oxidize ADHP forming an intense color or fluorescence measured at OD = 570 nm or at Ex/Em = 535/587 nm respectively.