Pyruvate Kinase Activity Assay, Catalog: MA-0107

Catalog MA-0107

Size 100 wells

Sample Type Tissue Extracts, Cell Lysate, Serum, Plasma, Urine, Other Biological Fluids

Species All

Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A plate-based colorimetric/fluorometric assay to measure pyruvate kinase activity in samples below 50 μU.

Sensitivity <50 μU

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Pyruvate kinase (PK) is a key enzyme in the glycolytic pathway catalyzing the conversion of phosphoenolpyruvate to pyruvate forming ATP. This reaction serves as a key control step regulating the overall rate of glycolysis and determining the fate of glucose metabolism. Pyruvate kinase plays a central role providing energy for cellular processes, as pyruvate is a metabolite common to several pathways. Pyruvate assay helps in understanding glycolytic flux, assessing enzyme regulation, and evaluating metabolic alterations in diseases including cancer, diabetes, or genetic disorders. AkrivisBio’s Pyruvate Kinase Activity Assay is a simple, sensitive assay for determining PK activity in a variety of sample types below 50 μU. In the assay, pyruvate kinase converts Phosphoenolpyruvate and ADP to generate pyruvate and ATP. Pyruvate is then oxidized by pyruvate oxidase, forming hydrogen peroxide. Hydrogen peroxide is utilized by peroxidase to oxidize ADHP resulting in the formation of intense color/fluorescence measured at OD 570 nm or at Ex/Em = 535/587 nm respectively.