Ammonia Assay II, Catalog: MA-0140

Catalog MA-0140

Size 100 wells

Sample Type Cell Lysate, Tissue Extracts, Urine, Serum, Plasma, Other biological fluids

Species All

Method of Detection Colorimetric, OD 670 nm

Assay Type Quantitative

Application A plate based colorimetric assay to measure the concentration of ammonia in various sample types.

Sensitivity <1 nmoles/100 μl well

Storage Conditions Room Temperature

Shipping Temperature Room Temperature

Shelf Life One year from the date of delivery

Ammonia is important both as a source of and a means of eliminating nitrogen in living systems. It is required for the synthesis of amino acids, as well as nucleotides and some lipids. Ammonia is typically formed through amino acid deamination. In the liver, ammonia is converted to the much less toxic urea and in birds and reptiles, into uric acid. AkrivisBio’s Ammonia Assay II is a simple, sensitive, reliable assay with a sensitivity below 1 nmole per 100 μl well. In the assay, ammonia is converted to indophenol, with a strong absorbance measured at 670 nm