Size Includes reagents to stain 96-, 48-, 24-, 12-, 6-well plates
Sample Type Cultured cells
Species All
Method of Detection Light Microscopy
Assay Type Qualitative
Application For visualizing ALP activity in cultured cells under a light microscope.
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Alkaline Phosphatase (ALP) is an enzyme found in various tissues throughout the body, including the liver, bone, kidney, and intestine. It plays a crucial role in catalyzing the hydrolysis of phosphate groups from various molecules including nucleotides and proteins. ALP is essential for many physiological processes, including bone mineralization, nucleic acid metabolism, and cellular signaling. AkrivisBio’s Alkaline Phosphatase utilizes the BCIP/NBT protocol based on indoxyl substrates. In the stain, BCIP is hydrolyzed to an indoxyl, which reduces the NBT to a darkly coloredformazan.
