ZnAF-2, 6-Iso, Catalog: 2756
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6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}fluorescein
6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}fluorescein
Alternate Names 6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}fluorescein
Appearance Crystalline solid
CAS # 321859-11-4
Molecular Formula C₃₄H₂₈N₄O₅
Molecular Weight 572.61
Purity ≥ 95% by HPCE
Solubility DMSO
SMILES C1=CC=NC(=C1)CN(CCNC2=CC(=C(C=C2)C(=O)O)C3=C4C=CC(=O)C=C4OC5=C3C=CC(=C5)O)CC6=CC=CC=N6
InChi InChI=1S/C34H28N4O5/c39-25-8-11-28-31(18-25)43-32-19-26(40)9-12-29(32)33(28)30-17-22(7-10-27(30)34(41)42)37-15-16-38(20-23-5-1-3-13-35-23)21-24-6-2-4-14-36-24/h1-14,17-19,37,39H,15-16,20-21H2,(H,41,42)
InChi Key CORIVNRIBACDOQ-UHFFFAOYSA-N
PubChem CID 16218345
MDL Number MFCD08277015
Storage Conditions -20°C
Shipping Conditions Gel Pack
Usage For Research Use Only! Not For Use in Humans.
Handling Do not take it internally. Wear a glove and mask when handling the product. Protect from air and moisture.
ZnAF-2, 6-iso-, is a cell-impermeable compound that acts as a high-affinity Zn²⁺-specific fluorescent probe (Kd = 2.7 nM). It shows low basal fluorescence, and the fluorescence intensity increases ~51-fold upon stoichiometric (1:1) binding to Zn²⁺. Exhibits little affinity towards Ca²⁺, Mg²⁺, Na⁺, or K⁺.