Branched Chain Amino Acid Assay, Catalog: MA-0157

Catalog MA-0157

Size 100 wells

Sample Type Tissue Extracts, Cell Lysate, Serum, Plasma, Urine, Other biological fluids

Method of Detection Colorimetric, OD 450 nm

Assay Type Quantitative

Application A plate based colorimetric assay to measure BCAA’s in a variety of sample types with a sensitivity down into the low nanomole range vs normal serum concentrations in the 50-300 nmole/ml range.

Sensitivity 0.2 nmoles/well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

The branched-chain amino acids, comprising leucine, isoleucine, and valine, are deemed essential, and must be obtained through diet as the body cannot produce them independently. BCAAs are particularly important for muscle health and growth. Leucine, in particular, acts as a powerful stimulator of protein synthesis and insulin secretion. BCAAs also act as an energy source during periods of intense exercise, preserving muscle mass and preventing their breakdown. BCAAs are unique in their ability to bypass the liver and be directly utilized by muscles for energy production. An imbalance in BCAA metabolism is linked to various health conditions like insulin resistance, obesity, and muscle wasting. Some of this appears to occur through mTORC impairment. On the other hand, maintaining an appropriate BCAA ratio is associated with improved insulin sensitivity and a reduced risk of chronic diseases. AkrivisBio’s BCAA Assay is a simple direct method of measuring the BCAA’s in a variety of sample types with a sensitivity down into the low nanomole range vs normal serum concentrations in the 50-300 nmole/ml range.