Phosphodiesterase Activity Assay, Catalog: MA-0160

Catalog MA-0160

Size 100 wells

Sample Type Tissue Extracts, Cell Lysate, Serum, Plasma, Urine, Other biological fluids

Method of Detection Fluorometric, Ex/Em = 370/450 nm

Assay Type Quantitative

Application A plate based fluorometric assay to measure PDE activity in a wide range of biological samples with a sensitivity into the low picomole/minute (µU) range.

Sensitivity Low picomole/minute (µU) range

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

The phosphodiesterase family of enzymes plays a significant role role in metabolism by regulating the levels of cyclic nucleotides, mainly cAMP and cGMP. These cyclic nucleotides are second messengers involved in a host of pathways which control many different physiological processes. PDEs catalyze the hydrolysis of cAMP and cGMP, inactivating them, leading to the termination of downstream signaling pathways. The influence of the PDE family is extensive and diverse. By controlling the levels of cAMP and cGMP, PDEs impact cell growth, differentiation, contractility, neurotransmission, and immune response. PDE Dysfunction has been linked to various diseases, including cardiovascular and neurological conditions, and cancer. PDE inhibitors have emerged as essential therapeutic agents to modulate cyclic nucleotide signaling in these diseases. The specificity and tissue distribution of the PDEs permits much flexibility in their inhibition. Different PDE isoforms exist in different cell types. PDE3 is found mainly in cardiac and vascular tissue, PDE 5 in smooth muscle cells. There are more than 20 isoforms of PDE4 alone. Understanding PDE function is important in many areas of biochemistry and medicine. AkrivisBio’s Phosphodiesterase Activity assay is a simple, sensitive means of measuring PDE activity in a wide range of biological samples with a sensitivity into the low picomole/minute (µU) range.