Nickel Assay, Catalog: MA-0170

Catalog MA-0170

Size 100 wells

Sample Type Variety of samples including Cell & Tissue Culture Supernatants, Urine, Plasma, Serum, as well as many other Biological Fluids.

Method of Detection Colorimetric, OD 330 nm & 405 nm

Assay Type Quantitative

Application A plate based simple, sensitive colorimetric assay to quantify Ni2+ in a variety of samples in the linear range of 2–50 nmole nickel in samples.

Sensitivity Linear range of 2-50 nmole nickel in samples

Storage Conditions Room Temperature

Shipping Temperature Room Temperature

Shelf Life One year from the date of delivery

Nickel is one of the most common of the metallic elements in the earth’s crust, 2nd only to iron. A number of enzymes utilize nickel as part of the active site, as a mononuclear (glyoxalase I, superoxide dismutase); dinuclear (urease); or more complex heteronuclear cluster (several hydrogenases and dehydrogenases). We take advantage of Nickel’s ability to form complexes with sulfhydryl compounds which possess absorbance bands in the UV/visible region. AkrivisBio’s Nickel Assay is a simple, sensitive way to quantify Ni2+ in a wide range of sample types. Since other ions also exhibit similar behavior with sulfur-containing chemicals, a differential absorbance method is used to correct for interfering substances.