Method of Detection Light and Fluorescence Microscopy (Ex/Em = 340-380/385-470 nm)
Assay Type Qualitative
Application A simple, sensitive histochemical method of localizing unesterified cholesterol in cells.
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Cholesterol is a vital and versatile structural component of eukaryotic cell membranes, with a fundamental role in maintaining membrane structural integrity and fluidity. Plasma membranes contain most of the cellular cholesterol with little present in the endoplasmic reticulum or mitochondrial membrane. Defects in intracellular cholesterol transport between compartments can alter cellular cholesterol metabolism resulting in pathological conditions such as atherosclerosis and lipid storage diseases. Filipin III selectively binds to and fluorescently labels cholesterol in membranes, emitting a blue fluorescence. AkrivisBio’s Cholesterol Detection Kit provides a simple, sensitive method of localizing unesterified cholesterol in cells.
