Phosphoglycerate Mutase Activity Assay, Catalog: MA-0209

Catalog MA-0209

Size 100 wells

Sample Type Cell Lysate, Tissue Extracts

Method of Detection Colorimetric, OD 570 nm

Assay Type Quantitative

Application A plate based simple, and sensitive means of assessing Phosphoglycerate Mutase  enzyme activity in a variety of sample types. 

Sensitivity In the low microunit range.  

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Phosphoglycerate mutase (PGAM) is a member of the glycolytic pathway, where it catalyzes the reversible conversion of 3-phosphoglycerate to 2-phosphoglycerate, a step occurring in both glycolysis and gluconeogenesis. During glycolysis, 3-PG is produced from phosphorylation of glyceraldehyde-3-phosphate. Phosphoglycerate mutase then converts 3-PG to 2-PG, allowing subsequent steps in glycolysis to proceed, maintaining the flow of metabolites through the glycolytic pathway, leading to production of pyruvate which enters the TCA cycle powering oxidative phosphorylation. In gluconeogenesis, 2-PG is converted back to 3-PG, an essential step for replenishment of glucose under conditions of low glucose levels, ensuring supply for tissues with high energy demands such as brain and red blood cells. Dysregulation of PGAM activity through mutation or expression is associated with several medical conditions. It may be upregulated in cancer cells which depend on a high glycolytic flux. AkrivisBio’s Phosphoglycerate Mutase Activity Assay is a simple, sensitive means of assessing enzyme activity in a variety of sample types with a sensitivity in the low microunit range.