Lactose Assay, Catalog: MA-0232

Catalog MA-0232

Size 100 wells

Sample Type Cell & Tissue culture supernatants, Urine, Plasma, Serum, Other Biological Fluids, Monitoring level during fermentation and feeding in protein expression processes and growth medium

Method of Detection Colorimetric 570 nm or Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A simple, sensitive method of determining lactose concentration in a variety of biological samples. 

Sensitivity Nanomole to picomole range

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Lactose, a disaccharide, primarily found in milk and dairy products, is composed of galactose and glucose in a β-1→4 linkage. Lactose metabolism occurs in the small intestine with the secretion of lactase or β-galactosidase to its constituent monosaccharides. Lactose intolerance is characterized by symptoms such as bloating, diarrhea and abdominal pain due to consumption of lactose by individuals with low lactase production. This is seen primarily in adults from Africa, and south and east Asia where consumption of dairy products is not a significant source of nutrition. Another rather rare disorder of galactose metabolism is galactosemia in which one of the enzymes in the Leloir pathway is deficient, usually due to genetic causes. Lactose enhances calcium absorption in the small intestine, enhancing bone health. Measurement of lactose in biological samples is thus an important capability. AkrivisBio Lactose Assay is a simple, sensitive method of determining lactose concentration in a variety of biological samples with a sensitivity in the nanomole to picomole range.