Fumarate Assay, Catalog: MA-0233

Catalog MA-0233

Size 100 wells

Sample Type Cell & Tissue culture supernatants, Urine, Plasma, Serum, Other Biological Fluids

Method of Detection Colorimetric 450 nm 

Assay Type Quantitative

Application A simple, sensitive means of measuring less than 1 nmole fumarate in a variety of biological samples. 

Sensitivity < 1 nmole fumarate

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Fumarate is an intermediate in the TCA cycle, formed from succinate by succinate dehydrogenase. Fumarate is subsequently hydrated to form malate. Fumarate also participates in the urea and purine nucleotide cycles. In the urea cycle it’s formed during conversion of arginosuccinate to arginine, linking nitrogen metabolism to energy production. Anaplerotic reactions which replenish TCA cycle intermediates, such as the breakdown of phenylalanine and tyrosine, also form fumarate. Hereditary Leiomyomatosis is a genetic condition caused by mutations in the fumarate hydratase gene. Mutations there lead to the accumulation of fumarate, which acts as an oncometabolite, inhibiting prolyl hydroxylase and stabilizing hypoxia-inducible factors. This stabilization promotes a pseudohypoxic response, driving tumorigenesis, particularly in the kidneys and smooth muscle tissues. Fumarate derivatives also act as immunomodulators. DMF is a treatment for multiple sclerosis, possibly activating the Nrf2 pathway. AkrivisBio’s Fumarate Assay is a simple sensitive means of measuring less than 1 nmole fumarate in a variety of biological samples.