Xanthine/Hypoxanthine Assay, Catalog: MA-0175

Catalog MA-0175

Size 100 wells

Sample Type Variety of samples including Serum, Plasma, Urine, Cell & Tissue Culture Supernatants

Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A plate based simple sensitive means of monitoring xanthine and hypoxanthine concentrations in a variety of sample types.

Sensitivity Mid micromolar range

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Xanthine and hypoxanthine are purine derivatives which are intermediates in the degradation of adenine and guanine. The purine catabolic pathway helps maintain the purine levels in the cell. They also participate in the purine salvage pathway, responsible for the recycling of nucleotides, necessary for replenishment of the cellular pool. Xanthine is also a phosphodiesterase inhibitor and an adenosine receptor antagonist. Adenosine promotes relaxation and sleep. Xanthine exerts a stimulant effect, enhancing alertness and reducing fatigue. Blood xanthine levels increase following ischemia, making xanthine a potentially useful marker for tissue hypoxia. AkrivisBio’s Xanthine/Hypoxanthine Assay is a simple sensitive means of monitoring xanthine and hypoxanthine concentrations in a variety of sample types with a sensitivity in the mid micromolar range.