100 wells, Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm
PRODUCT SUMMARY
Catalog MA-0174
Size 100 wells
Sample Type Cell & Tissue Culture Lysates
Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm
Assay Type Quantitative
Application A plate based simple, sensitive to measure enolase activity in a variety of biological sample types.
Sensitivity Low μU range
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Enolase plays a central role in glycolysis. It converts 2-phosphoglycerate to phosphoenolpyruvate. It also catalyzes the reverse reaction (PEP to 2-phosphoglycerate) during gluconeogenesis. Enolase has been found in every organism with a glycolytic pathway. Other than its role in glycolysis, enolase has been found to affect other functions such as transcriptional regulation, cell adhesion and stressor response. Extracellular enolase interacts with the extracellular matrix and proteins like laminin and fibronectin. Cancer cells may upregulate enolase to enhance their adhesion to the ECM, facilitating metastasis. Enolase also bind plasminogen, activating it as plasmin which can degrade ECM components AkrivisBio’s Enolase Activity Assay is a simple sensitive way to measure enolase activity in a variety of biological sample types with a sensitivity in the low μU range.
