Chymotrypsin Activity Assay, Catalog: MA-0181

Catalog MA-0181

Size 100 wells

Sample Type Cell & Tissue Culture Supernatants, Urine, Plasma, Serum, Other Biological Fluids

Method of Detection Fluorometric, Ex/Em = 380/460 nm

Assay Type Quantitative

Application A plate based simple, sensitive means of measuring chymotrypsin activity in a range of biological samples.

Sensitivity Low microunit ranges

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Chymotrypsin is a serine protease with a substrate specificity which targets peptide bonds on the carboxyl side of large hydrophobic amino acids like tyrosine, tryptophan and phenylalanine. Primarily produced by the pancreas, but also expressed in spleen and liver, chymotrypsin is generally secreted into the gut where it aids in the digestion of dietary protein. Conditions which interfere with its release, such as cystic fibrosis or cancerous tumors, which can obstruct pancreatic ducts and can have severe detrimental effects on health and well-being. Pancreatitis can involve the inappropriate activation of chymotrypsinogen leading to autodigestion of pancreatic tissue. AkrivisBio’s Chymotrypsin Activity Assay is a simple, sensitive way to measure chymotrypsin activity in a range of biological sample types with a sensitivity limit in the low microunit range.