Neutral Red Cell Cytotoxicity Assay, Catalog: CPT-0107

Catalog CPT-0107

Size Includes reagents for 1000 wells

Sample Type Cells

Species Mammalian

Method of Detection Colorimetric, OD 540 nm

Assay Type Quantitative

Application A convenient and non-radioactive alternative for assessing cell viability and cytotoxicity in vitro.

Storage Conditions 4°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

The neutral red cytotoxicity assay is a widely used method for assessing cell viability and cytotoxicity in vitro. This assay is based on the ability of viable cells to incorporate and retain the neutral red dye within lysosomes. The principle behind this technique relies on the fact that only living cells can actively transport and accumulate the dye, while dead or damaged cells cannot. The neutral red assay offers several advantages over other cytotoxicity tests. It is more sensitive than many other cytotoxicity assays, such as those based on tetrazolium salts or enzyme leakage; the test is less inexpensive than other methods; it can be used with a wide range of cell types, including primary cells and various cell lines; it’s a relatively quick procedure. Once the cells have been treated, the assay can be completed in less than 3 hours. The limitations of this assya are that colored compounds may interfere with the spectrophotometric measurements, and careful attention must be paid to avoid crystal formation of the neutral red dye during the protocol. A notable point is the inclusion of the Neytral Red Assay in regulatory guidelines. The 3T3 Neutral Red Uptake (NRU) phototoxicity assay has been accepted by the European Union and OECD member states as a test guideline (TG 432) for assessing the phototoxic potential of chemicals. AkrivisBio’s Neutral Reed Assay is a valuable tool due to its simplicity, sensitivity and broad applicability.