Aldehyde Dehydrogenase Activity Assay II (F), Catalog: MA-0248

Catalog MA-0248

Size 100 wells

Sample Type Cell & Tissue Culture Lysates, Plasma, Serum, Other Biological Fluids.

Method of Detection Fluorometric, Ex/Em =  535 nm/ 587 nm

Assay Type Quantitative

Application A highly sensitive, fluorescence-based assay to detect ALDH activity in various biological samples.

Sensitivity < 25 µUnits per sample

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Aldehyde Dehydrogenase (ALDH) encompasses a number of related enzyme activities. The human genome contains 19 functional genes, each with its own specific role and tissue distribution. ALDH roles include detoxification of reactive aldehydes, alcohol metabolism, retinoic acid biosynthesis, folate metabolism, neurotransmitter metabolism and lipid peroxidation. Important conditions related to aldehyde metabolism include cancer where heightened ALDH expression is linked to various cancers and chemotherapy resistance. Conversely, ALDH deficiency increases the risk of esophageal, oropharyngolaryngeal, and other cancers due to acetaldehyde accumulation. Mutations in ALDH3A2 cause Sjögren-Larsson syndrome characterized by ichthyosis, spastic diplegia, and intellectual disability. ALDH4A1 deficiency leads to proline accumulation and neurological symptoms. ALDH5A1 mutations cause γ-hydroxybutyric aciduria and altered GABA metabolism. AkrivisBio’s Aldehyde Dehydrogenase Assay II is a highly sensitive, fluorescence-based assay with the ability to detect activity at levels below 25 µUnits per sample.