Aromatase (CYP19A) Activity Assay, Catalog: MA-0250

Catalog MA-0250

Size 100 wells

Sample Type Tissue Lysates, Cell Lysates, Human Liver Microsomes, Liver S9 fractions, Primary Hepatocytes, Heterologously Expressed Recombinant Aromatase Preparations

Method of Detection Fluorometric, Ex/Em = 490/535 nm

Assay Type Quantitative

Application A simple, sensitive way to quantitate Aromatase enzyme activity in a variety of biological sample types with a sensitivity in the low nano-Unit range.

Sensitivity In the low nano-Unit range

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

CYP19A is responsible for the biosynthesis of estrogens from androgens. It is expressed in multiple tissues, including gonads, placenta, brain, adipose tissue and bone, allowing for localized estrogen production. Aromatase activity is tightly regulated by tissue-specific promoters, controlled by various hormones and cytokines. This complex regulation allows for fine-tuned estrogen production in different tissues. Instances of CYP19A influence on metabolism are in breast cancer, osteoporosis, gynecomastia and hypogonadism and in obesity and metabolic syndrome. Inhibitors of CYP19A are finding increased use for a variety of medical conditions. AkrivisBIo’s Aromatase Activity Assay is a simple, sensitive way to quantitate enzyme activity in a variety of biological sample types with a sensitivity in the low nano-Unit range.