Cobalt Assay, Catalog: MA-0258

Catalog MA-0258

Size 100 wells

Sample Type Cell & Tissue Lysates, Urine, Plasma, Serum, Other Biological Fluids

Method of Detection Colorimetric, OD 475 nm

Assay Type Quantitative

Application A plate-based colorimetric assay to measure cobalt with a sensitivity approaching 1 nanomole of cobalt.

Sensitivity Approaching 1 nanomole of cobalt

Storage Conditions Room Temperature

Shipping Temperature Room Temperature

Shelf Life One year from the date of delivery

Cobalt, a hard, lustrous, grey metal, symbol Co, atomic number 27, atomic mass 58.9 is an essential trace-element for all multicellular organisms as the active center of cobalamin (vitamin B12). Cobalamin is a cofactor for methylmalonyl-CoA mutase and methionine synthase, critical for DNA and amino acid synthesis, fatty acid metabolism and energy production. Other important uses for cobalt include hematopoiesis, maintenance of the nervous system, myelin synthesis and as a component of HIF, a transcriptional activator which stimulate erythropoietin. Cobalt deficiency leads to dysfunction of methionine synthase and methylmalonyl-CoA mutase, megaloblastic anemia and nervous system disorders. In biotechnology cobalt is one of the most important metal ions used in protein purification protocols using His-Tag protocols. AkrivisBio’s Cobalt Assay is a very simple, add-and-read procedure for measurement of cobalt with a sensitivity approaching 1 nanomole of cobalt.