Dihydrofolate Reductase Activity Assay, Catalog: MA-0264

Catalog MA-0264

Size 100 wells

Sample Type Cell & Tissue lysate, Purified Recombinant Protein

Method of Detection Colorimetric, OD 340 nm

Assay Type Quantitative

Application A plate-based simple, sensitive colorimetric assay designed to measure Dihydrofolate Reductase activity in biological samples.

Sensitivity Near 1 mU

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Dihydrofolate Reductase catalyzes the reduction of dihydrofolate to tetrahydrofolate using NADPH as the electron donor. Tetrahydrofolate is essential as a cofactor for several enzymes responsible for the synthesis of purines, thymidylic acid, and some amino acids, making DHFR indispensable for DNA synthesis and cell proliferation. DHFR is present in most tissues and cells with activity peaking at the G1/S boundary. In humans, there are two genes: DHFR and DHFR2/DHFRL1, with the latter targeted to mitochondria. Interestingly, mammalian DHFR can also reduce folic acid to THF, allowing for the use of folic acid in food supplementation. DHFR inhibitors are used in several therapeutic interventions, including cancer treatment, antibiotics and antiparasitic drugs. AkrivisBio’s Dihydrofolate Reductase Activity Assay is a sensitive, straightforward way of measuring DHFR activity in biological samples with a sensitivity near 1 mU.