Enteropeptidase/Enterokinase Activity Assay, Catalog: MA-0270

Catalog MA-0270

Size 100 wells

Sample Type Biological Samples, Purified Enteropeptidase Protein

Method of Detection Fluorometric, Ex/Em = 380/500 nm

Assay Type Quantitative

Application A plate-based simple, sensitive fluorometric assay to measure Enteropeptidase/Enterokinase enzyme activity in a variety of samples.  

Sensitivity Approaching 1 µUnit.

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Enteropeptidase, also known as Enterokinase, is a member of the serine protease family, localized to the brush border of the duodenal and jejunal mucosa. Its primary function is to initiate intestinal digestion by activating trypsinogen to its active form, trypsin. This initiates a cascade of enzymatic reactions, activating other proenzymes to their active forms including chymotrypsin, elastase, carboxypeptidase and lipase. Enteropeptidase has a very specific substrate recognition motif of Asp-Asp-Asp-Asp-Lys (DDDDK). Due to its broad involvement in digestion, it has become a target of interest for pharmaceutical companies. AkrivisBio’s Enteropeptidase/Enterokinase Activity Assay is a simple, sensitive way to measure enzyme activity in a variety of samples with a sensitivity approaching 1 µUnit.