Galactose and Lactose Assay, Catalog: MA-0280

Catalog MA-0280

Size 100 wells

Sample Type Cell & Tissue culture supernatants, Urine, Plasma, Serum, Other Biological Fluids, Monitoring Levels During Fermentation & Feeding In Protein Expression Processes.

Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A plate-based simpl, sensitive assay to quantify Galactose and Lactose in a variety of sample types. 

Sensitivity < 1 nanomole colorimetrically & < 100 picomoles fluorometrically. 

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Lactose and galactose play important metabolic roles in human metabolism, particularly in early development and energy production. Lactose, a disaccharide found primarily in milk and dairy products, consists of glucose and galactose. Upon ingestion, lactose is hydrolyzed into these monosaccharides, which are absorbed and metabolized. Galactose is converted to glucose through the Leloir pathway. This conversion is essential since the human body cannot directly use galactose for energy production. Lactose serves several functions. As a carbohydrate, lactose provides energy, particularly for infants who rely heavily on milk for nutrition; Lactose has a low glycemic index; Undigested lactose that reaches the lower intestine can influence the gut microbiome. Galactose on the other hand, is converted to glucose-6-phosphate (LeLoir pathway); is a vital precursor for the synthesis of glycoproteins, glycolipids, and proteoglycans and is suggested to have a beneficial effect on brain health and function. AkrivisBio’s Galactose and Lactose Assay is a simple sensitive way to quantify each of these sugars in a variety of sample types with a sensitivity less than 1 nanomole colorimetrically and less than 100 picomoles fluorometrically.