Glucose-6-Phosphate Dehydrogenase Activity Assay (C), Catalog: MA-0291

Catalog MA-0291

Size 100 wells

Sample Type Tissue Lysates, Cell Extracts, Serum, Urine, Plasma, Other biological fluids.

Method of Detection Colorimetric, OD 450 nm

Assay Type Quantitative

Application A plate-based simple, sensitive and rapid colorimetric assay to detect the activity of Glucose-6-Phosphate Dehydrogenase in a variety of samples.

Sensitivity As low as 0.04 mU G6PDH per well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose  phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance  is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. BioVision's glucose-6-phosphate dehydrogenase Assay Kit is a simple, sensitive and rapid assay detects the activity of G6PDH in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product which is utilized to convert a nearly colorless probe to an intensely colored product with an absorbance at 450 nm. The G6PDH Assay Kit can detect as low as 0.04 mU G6PDH per well.