Method of Detection 100 wells, Fluorometric, Ex/Em = 390/480 nm
Assay Type Quantitative
Application A simple minimally invasive way to monitor intracellular Reduced glutathione levels in live cells.
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Reduced glutathione (GSH) is the major low molecular-weight thiol in living cells with an intracellular concentration ranging from 0.5 to 15 mM. As a highly reactive species, it forms conjugates with electrophilic molecules, increasing their aqueous solubility, allowing for more rapid elimination. Oxidative stress due to lowered levels of GSH results from consumption occurring faster than replacement by glutathione reductase. This results in many forms of cellular and mitochondrial damage which if severe enough leads to cell death. A variety of compounds can affect the intracellular glutathione level of cells. AkrivisBio’s Cell-Based Glutathione Detection Kit (Blue Fluorescence) is a simple minimally invasive way to monitor intracellular GSH levels in live cells.
