Total Glutathione Assay (F), Catalog: MA-0296

Catalog MA-0296

Size 100 wells

Sample Type Cell & Tissue lysates, Culture Media, Urine, Plasma, Serum, Other Biological Fluids

Method of Detection Fluorometric, Ex/Em = 360/460 nm

Assay Type Quantitative

Application A plate-based simple, sensitive Fluorometric assay to measure total cellular glutathione in a variety of biological samples.

Sensitivity < 1 µg/ml liquid or < 10 ng/mg tissue 

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Glutathione (GSH) is a tripeptide antioxidant that is involved in numerous metabolic processes within the body. GSH serves as a powerful antioxidant, reducing oxidative stress by neutralizing reactive oxygen species. It directly reduces peroxides and free radicals, converting them into less harmful compounds. It is also conjugated to various toxins by cellular glutathione transferases, accelerating their elimination. GSH protects and regulates the redox state of proteins by S-glutathionylation which helps protect protein thiols from irreversible oxidation and modulates protein function. It enhances lymphocyte proliferation, protects mitochondria and helps maintain the reduced state of other antioxidants like ascorbate and vitamin E. AkrivisBio’s Total Glutathione Assay is a simple sensitive way to measure total cellular glutathione with a sensitivity below 1 µg/ml liquid or 10 ng/mg tissue.