Isocitrate Assay, Catalog: MA-0306

Catalog MA-0306

Size 100 wells

Sample Type Cell & Tissue Lysates, Urine, Pasma, Serum, Other Biological Fluids

Method of Detection Colorimetric, OD 450 nm

Assay Type Quantitative

Application A plate-based simple, sensitive colorimetric assay to measure isocitrate in a variety of samples.

Sensitivity < 0.5 nanomoles per well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

The conversion of isocitrate to α-ketoglutarate is a major regulatory step of the TCA cycle and has profound implications for energy metabolism, redox balance and many biosynthetic pathways. NADH or NADPH are both formed by different isoforms of isocitrate dehydrogenase. While NADH is important for energy metabolism, NADPH is important for redox balance, keeping glutathione in the reduced state. Isocitrate provides a carbon skeleton for nitrogen assimilation in plants, supports the glyoxalate cycle and has a role in glucose sensing and insulin secretion in pancreatic β cells. AkrivisBio’s Isocitrate Assay is a simple, sensitive way to measure isocitrate with a sensitivity below 0.5 nanomoles per well.