Isocitrate Dehydrogenase Activity Assay, Catalog: MA-0307

Catalog MA-0307

Size 100 wells

Sample Type Cell & Tissue Lysates, Urine, Plasma, Serum, Other Biological Fluids

Method of Detection Colorimetric, OD 450 nm

Assay Type Quantitative

Application A plate-based colorimetric assay to measure Isocitrate Dehydrogenas activity in a variety of samples.

Sensitivity < 10 µUnits per well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Isocitrate dehydrogenase catalyzes the oxidative decarboxylation of isocitrate to α-ketoglutarate, producing NADH or NADPH with some isoforms and CO₂ as byproducts. This reaction is a key regulatory step in the TCA cycle, linking energy production with biosynthetic processes. NADH is used in the electron transport chain to produce energy, NADPH is used for maintaining redox balance through recharging GSH stocks and ketoglutarate is used in a variety of synthetic pathways. Mutations of IDH are implicated in several cancers.. AkrivisBio’s Isocitrate Dehydrogenase Activity Assay is a simple, sensitive way to measure IDH activity in a variety of samples with a sensitivity below 10 µUnits per well.