Method of Detection Fluorometric, Ex/Em = 529/600 nm
Assay Type Quantitative
Application A plate based fluorometric assay for measuring Lipase enzyme activity in various samples.
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Lipases play a pivotal role in digestion, absorption, and metabolism of dietary fats in organisms. In the human digestive system, lipases break down triglycerides into fatty acids and glycerol, enabling efficient nutrient absorption in the intestine. Lipase is a key regulator of energy balance. By releasing fatty acids from adipose tissue during periods of energy demand, lipases enable the body to access stored energy reserves. This is particularly crucial during fasting or physical activity when glucose availability is limited. Lipase activity influences lipid-related disorders such as obesity, diabetes, and cardiovascular diseases. Dysregulation of lipase function can lead to abnormal lipid profiles and contribute to the development of these conditions. AkrivisBio’s Lipase Activity Assay III is directed towards lipases which digest triglycerides. Lipase activity results in a dramatic increase in fluorescence observed permitting detection of lipase activity below 5 µUnits per well.
