Sample Type Purified Protein, Cell Extracts, Cell Media
Method of Detection Fluorometric, Ex/Em = 535/587 nm
Assay Type Quantitative
Application A plate-based fluorometric assay to measure Lysyl Oxidase activity in various samples.
Sensitivity < 5 µUnits per well
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Lysyl oxidase (LOX) is an enzyme that has a role in the formation and maintenance of the extracellular matrix with significant implications for metabolic health. A copper-dependent amine oxidase, LOX is primarily secreted by smooth muscle cells and fibroblasts, catalyzing the crosslinking of collagen and elastin fibers. Initially recognized for its structural functions, recent research has unveiled correlations between LOX activity and various physiological and pathological processes, including adipose tissue function, glucose metabolism and type 2 diabetes, fibrosis in several tissues and cardiovascular health. Inhibition of LOX has shown promise in animal models for reducing metastasis and improving metabolic outcomes but given the essential nature of LOX in normal tissue development and function, consideration must be given to potential side effects of LOX modulation. AkrivisBio’s Lysyl Oxidase Activity Assay is a simple sensitive means of measuring LOX activity with a sensitivity below 5 µUnits per well.
