Malate Dehydrogenase Activity Assay, Catalog: MA-0316

Catalog MA-0316

Size 100 wells

Sample Type Tissue or Cell Lysate, Mitochondria

Method of Detection Colorimetric, OD 450 nm

Assay Type Quantitative

Application A plate based simple, sensitive colorimetric assay to measure Malate Dehydrogenase activity in various sample types.

Sensitivity < 0.5 mU of MDH activity

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malate/aspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma). In AkrivisBio’s Malate Dehydrogenase Activity Assay Kit, MDH reacts with malate to form an intermediate. The generated intermediate reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.