Succinyl-CoA Synthetase Activity Assay, Catalog: MA-0351
US$440.00
100 wells, Colorimetric, OD 450 nm
100 wells, Colorimetric, OD 450 nm
Catalog MA-0351
Size 100 wells
Sample Type Cell & Tissue Lysates, Urine, Plasma, Serum, Other biological fluids
Method of Detection Colorimetric, OD 450 nm
Assay Type Quantitative
Application A plate-based simple, sensitive assay to quantitate Succinyl-CoA Synthetase activity in a variety of sample types. activity.
Sensitivity < 100 µU of activity
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
Succinyl-CoA Synthetase (SCS) also known as succinate thiokinase or succinyl-CoA ligase, is important in cellular metabolism with significant roles in energy production and multiple pathways. It catalyzes the reversible conversion of succinyl-CoA to succinate, coupled with the production of either ATP or GTP, depending on the isoform. In the citric acid cycle, it facilitates substrate-level phosphorylation, generating ATP or GTP directly. The enzyme's ability to produce ATP via substrate-level phosphorylation is particularly crucial in anaerobic environments, making it the only mitochondrial enzyme capable of ATP production without oxygen. SCS serves as a critical link between various metabolic pathways: It provides an entry point for the breakdown products of isoleucine, threonine, methionine, and valine into the citric acid cycle; it facilitates the entry of odd-chain fatty acid catabolism products into the TCA cycle; It is involved in ketone body breakdown, highlighting SCS's role in energy metabolism during fasting or ketogenic states. Succinyl CoA is a key intermediate in porphyrin and thus, heme biosynthesis. AkrivisBio’s Succinyl CoA Synthetase Activity Assay is a simple, sensitive way to quantitate SCS activity in a variety of sample types with a sensitivity below 100 µU of activity.