HDAC6 Activity Assay (F), Catalog: MA-0330

Catalog MA-0330

Size 100 wells

Sample Type Drugs, Inhibitors or Ligands (compounds that can interact and affect HDAC6 activity)

Method of Detection Fluorometric, Ex/Em = 375/480 nm

Assay Type Quantitative

Application A plate-based fluorometric assay to measure Histone deacetylase 6 activity in a variety of samples. 

Storage Conditions -80°C

Shipping Temperature Dry Ice

Shelf Life One year from the date of delivery

Histone deacetylase 6 (HDAC6) is a unique member of the HDAC family, primarily localized in the cytoplasm, that plays a significant role in various metabolic processes. Its importance is due to its ability to regulate multiple cellular functions. While normally associated with ϵ-amino deacetylation of histones, it has other important functions as well. HDAC6 is an essential regulator of hepatic glucocorticoid-stimulated gluconeogenesis. HDAC6 deficiency impairs dexamethasone-induced hepatic glucocorticoid receptor translocation, leading to attenuated expression of gluconeogenic genes. HDAC6 inhibitors, such as tubastatin A, have demonstrated potent anti-obesity effects in diet-induced obese mice. These inhibitors reduce food intake, fat mass, and hepatic steatosis while improving systemic glucose homeostasis. It is also  involved in lipid metabolism, cellular stress response and cytoskeletal regulation through α-tubulin modification. AkrivisBio’s HDAC6 Activity Assay is a simple sensitive way to measure HDAC6 activity in a variety of samples.