Transketolase Activity Assay, Catalog: MA-0202

Catalog MA-0202

Size 100 wells

Sample Type Tissue Lysate, Cell Lysate, Purified Protein, Recombinant enzyme

Method of Detection Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A plate based simple and sensitive fluorimetric assay to measure Transketolase activity in a variety of tissue samples.

Sensitivity < 5 µU

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Transketolase sits at the intersection of the pentose phosphate pathway and glycolysis and participates in the Calvin Cycle. It reversibly transfers a 2-carbon fragment from xylulose-5-P onto either ribose-5-P forming sedoheptulose-7-P and glyceraldehyde-3-P or to erythrose-4-P forming fructose-6-P and glyceraldehyde-3-P. This process is a significant source of NADPH and is important for maintaining the redox state of the cell. The reverse reaction is crucial for formation of  ribose-5-phosphate. Transketolase pays a significant role in the Warburg effect and is associated with increased metabolism in cancer, where inhibition promotes tumor cell apoptosis. It appears to play a role in lipid metabolism and in neurological health. AkrivisBio’s Transketolase Assay is a simple sensitive way to measure Transketolase activity in a variety of tissue samples with a sensitivity below 5 µU.