TIBC / Serum Iron Assay (C), Catalog: MA-0372

Catalog MA-0372

Size 100 wells

Sample Type Serum, Plasma. Serum-off-the clot is preferable to normal serum

Method of Detection Colorimetric, OD 570 nm

Assay Type Quantitative

Application A simple way to determine TIBC (total iron binding sites), serum iron concentration, transferrin bound iron and/or % saturation of transferrin in serum samples. 

Sensitivity TIBC typically varies between ~40-80 µM (2.4-4.5 mg/L and Serum Iron between 10 and 30 µM (0.5-1.75 mg/L).

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

A TIBC / Serum Iron assay measures the % saturation of transferrin in serum. In the assay, an iron solution is added to one of two serum samples. Excess iron is removed, and the remaining iron is measured. The difference between the two denotes the amount of available iron binding sites there are in the sample. One of two more samples is treated to release any iron bound to transferrin then the two samples are measured. The difference is the amount of iron bound to transferrin. The ratio of iron bound to transferrin/the total available binding sites gives the % of transferrin sites binding iron or % saturation. AkrivisBio’s TIBC / Serum Iron Assay is a simple way to determine TIBC (total iron binding sites), serum iron concentration, transferrin bound iron and/or % saturation of transferrin in serum samples. TIBC typically varies between ~40-80 µM (2.4-4.5 mg/L and Serum Iron between 10 and 30 µM (0.5-1.75 mg/L).