Sample Type Purified Gelatinase, Cell Lysates, Tissues Lysates
Method of Detection Fluorometric, Ex/Em = 490 nm/520 nm
Assay Type Quantitative
Application A simple way to to assess gelatinase activity in a variety of sample types.
Sensitivity < 10 µCDU
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Gelatinases, a subgroup of matrix metalloproteinases, play a role in the degradation of extracellular matrix components and tissue remodeling. They consist primarily of two zinc-dependent enzymes: gelatinase A (MMP-2) and gelatinase B (MMP-9). They break down gelatin, a denatured from of collagen and other ECM proteins such as type IV collagen, a component of basement membranes. Their activity is regulated at various levels, including gene expression, proenzyme activation, and inhibition by tissue inhibitors of metalloproteinases. They are produced as inactive proenzymes and activated in the extracellular space. Gelatinases affect tissue restructuring during development, wound healing, and angiogenesis; cell movement, important in processes like embryonic development and immune cell trafficking; formation of new blood vessels by degrading the ECM and releasing growth factors; modulation of the inflammatory response. AkrivisBio’s Gelatinase Assay is a simple way to assess gelatinase activity in a variety of sample types with a sensitivity below 10 µCDU.
